Testing Services

The Nano Characterization Laboratory (NCL)

NCL is responsible for providing testing services both for internal and external clients. The lab  is equipped with the state-of-the-art equipments for testing and analysis in nano-scale measurements and characterization.  A team of experts and well-trained technicians are there to provide services of fast response, high precision, and high quality standard. In 2011, the lab performed over 6000 technical testing services.

Nano-analytical equipments :

• Atomic Force Microscope

Atomic force microscopy
(AFM) or scanning force microscopy (SFM) is a very high-resolution type of scanning probe microscopy, with demonstrated resolution on the order of fractions of a nanometer, more than 1000 times better than the optical diffraction limit.
AFM has several advantages. It provides a three-dimensional surface profile. Additionally, samples viewed by AFM do not require any special treatments (such as metal/carbon coatings) that would irreversibly change or damage the sample. Most AFM modes can work perfectly well in ambient air or even a liquid environment. This makes it possible to study biological macromolecules and even living organisms. In principle, AFM can provide higher resolution than SEM. It has been shown to give true atomic resolution in ultra-high vacuum (UHV) and, more recently, in liquid environments. High resolution AFM is comparable in resolution to scanning tunneling microscopy and transmission electron microscopy.

• E-SEM (Environment Scanning Electron Microscope)
E-SEM or environmental scanning electron microscope is a scanning electron microscope (SEM) that allows a gaseous environment in the specimen chamber. Whereas all other electron microscopes operate in vacuum, the ESEM has added a new dimension to electron microscopy with enormous advantages.

• STM (Scanning Tunneling Microscope)
A scanning tunneling microscope (STM) is a powerful instrument for imaging surfaces at the atomic level. For an STM, good resolution is considered to be 0.1 nm lateral resolution and 0.01 nm depth resolution. With this resolution, individual atoms within materials are routinely imaged and manipulated. The STM can be used not only in ultra high vacuum but also in air, water, and various other liquid or gas ambients, and at temperatures ranging from near zero kelvin to a few hundred degrees Celsius.
The STM is based on the concept of quantum tunneling. When a conducting tip is brought very near to the surface to be examined, a bias (voltage difference) applied between the two can allow electrons to tunnel through the vacuum between them. The resulting tunneling current is a function of tip position, applied voltage, and the local density of states (LDOS) of the sample. Information is acquired by monitoring the current as the tip’s position scans across the surface, and is usually displayed in image form. STM can be a challenging technique, as it requires extremely clean and stable surfaces, sharp tips, excellent vibration control, and sophisticated electronics.

• MTT Assay
The MTT assay is colorimetric assay for measuring the activity of enzymes that reduce MTT or close dyes (XTT, MTS, WSTs) to formazan dyes, giving a purple color. A main application allows assessing the viability (cell counting) and the proliferation of cells (cell culture assays). It can also be used to determine cytotoxicity of potential medicinal agents and toxic materials, since those agents would stimulate or inhibit cell viability and growth.

• UV-Visible Spectrophotometer
Ultraviolet-visible spectrophotome
ter refers to absorption spectroscopy in the ultraviolet-visible spectral region. This means it uses light in the visible and adjacent (near-UV and near-infrared (NIR)) ranges. The absorption in the visible range directly affects the perceived color of the chemicals involved. In this region of the electromagnetic spectrum, molecules undergo electronic transitions. This technique is complementary to fluorescence spectroscopy, in that fluorescence deals with transitions from the excited state to the ground state, while absorption measures transitions from the ground state to the excited state.
UV/Vis spectroscopy is routinely used in the quantitative determination of solutions of transition metal ions and highly conjugated organic compounds.

• Freeze Dryer
uses the dehydration process to preserve a perishable material or make the material more convenient for transport. It works by freezing the material and then reducing the surrounding pressure and adding enough heat to allow the frozen water in the material to sublime directly from the solid phase to the gas phase.
Freeze-dryer are used in various applications such as pharmaceutical and biology, food sector, and technology sector.
There are essentially three categories of freeze-driers: the rotary evaporator freeze-drier, the manifold freeze-drier, and the tray freeze-drier.

For Service Request and Charge fee, please review the forms and contact Tel.0 2564 7100 : 6643 , 6567 fax: 0 2564 6981 E-mail: csa@nanotec.or.th

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Nano safety and risk assessment Lab (SRA)

SRA conducts the research to address possible adverse effects of nanomaterials and novel substances on human health and the environment. The methodology for safety investigation are thoroughly implemented case-by-case, using the available guidelines and standards, as well as alternative scientific procedures for the best practice. The group is currently focusing on development of reliable in vitro models for determining toxicological effects and pharmacological properties of materials upon their routes of exposure. SRA also introduces models of zebrafish and microorganisms into our research studies and testing services.

For Service Request and Charge fee, please review the forms and contact Tel.0 2564 7100 : 6643 , 6567 fax: 0 2564 6981 E-mail: csa@nanotec.or.th